FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238426
Sequences flagged as poor quality0
Sequence length91
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA18120.7599842299078121No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.4768775217467894No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA9580.40180181691594036No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA9480.397607643461703No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.38250861902644845No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.3695066813183126No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.2881397163061075No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT6270.2629746755806833No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA5800.24326206034576767No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.20090090845797018No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA4150.174058198350851No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3970.16650868613322373No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.1593785912610202No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.140504810716952No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC3190.13379413319017222No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA3070.12876112504508738No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC3020.12666403831796869No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.11617860468237524No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA2670.11198443122813788No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA2650.11114559653729039No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.11072617919186666No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC2530.10611258839220555No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA2500.10485433635593433No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.1044349190105106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAT150.001028562584.999798
GTGGTAT4200.067.8116761
GCGTCAG454.4025182E-756.6665274
TGGTATC5550.052.071942
TAACTGG250.00778879650.9998744
GAATAAG703.441528E-948.5814971
TAAGGAC354.8193027E-448.571314
GTATCAA26000.044.6342541
AGCGTCA603.1981745E-642.4998933
CGACTTT1500.042.4998933
AATAAGC701.8906394E-742.4998932
GGTATCA11600.041.0429881
TATCAAC29450.040.263062
ATAAGCT951.1368684E-940.2630583
CCCTTGA753.2439675E-739.674891
ACAATAT450.00166042437.7776833
GTTATCA903.173409E-837.7776838
CGTCAGA709.217651E-636.4284825
TCAACGC33550.035.469364
CAACGCA33750.035.2591745
Produced by FastQC (version 0.11.5)