FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51664
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA8291.604598947042428No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA4170.8071384329513782No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.6832610715391763No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.5264787860018582No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA2690.5206720346856611No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.4509910188912976No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.449055435119232No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.36388974914834316No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA1530.2961443171260452No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1430.2767884794053887No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA1330.2574326416847321No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.24194797150820685No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.20710746361102508No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.18581604211830288No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.1800092908021059No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA840.16258903685351503No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT830.16065345308144938No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG830.16065345308144938No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.15871786930938372No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA800.1548467017652524No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA740.14323319913285848No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.1412976153607928No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA730.1412976153607928No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.13936203158872715No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA720.13936203158872715No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.1374264478166615No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.12774852895633323No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.12581294518426756No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA650.12581294518426756No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.12581294518426756No Hit
TTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATAC650.12581294518426756No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTG620.12000619386807061No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTG610.11807061009600495No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG600.1161350263239393No Hit
TTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.11032827500774232No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.11032827500774232No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC560.10839269123567667No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10645710746361103No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC550.10645710746361103No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC540.10452152369154538No Hit
GATATACGCATTGTGGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.1025859399194797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1800.066.111111
TGGTATC1900.064.868422
AGCGTCA250.007754411551.03
GACCAGT250.007754411551.01
TCAAGAC250.007754411551.04
GCGTCAG250.007754411551.04
GTATCAA8750.042.7428551
GATATAC5250.041.2857131
TATCAAC9150.040.8743172
ATATACG5450.039.7706382
TAACCTT559.435634E-538.6363686
TATACGC5700.038.0263143
ATACGCA5800.037.3706934
GGTATCA4200.036.428571
ACGCATT5900.036.016956
CGCATTG5900.036.016957
TACGCAT5950.035.7142875
GCATTGT6000.035.4166648
ATCAACG10600.034.8820723
TCAACGC10650.034.718314
Produced by FastQC (version 0.11.5)