FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145047
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA11340.7818155494425945No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA6240.43020538170386147No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.3991809551386792No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.3467841458285935No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA4870.33575323860541756No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.2909401780112653No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT3630.2502637076258041No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA3290.2268230297765552No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.2219970078664157No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.2054506470316518No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2660.18338883258529995No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA2270.15650099622880861No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA2170.14960667921432363No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC1960.13512861348390523No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA1920.13237088667811125No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.13168145497666273No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.12202941115638379No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.12065054775348681No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA1670.11513509414189883No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC1660.11444566244045035No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC1630.11237736733610486No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA1520.10479361862017139No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATC259.125717E-567.9997257
GTAGACT200.003217854363.749744
GCGTCAG554.0381565E-1061.817934
GTGGTAT3300.057.97431
GTATCAC302.2448567E-456.6859861
TGGTATC3450.055.434562
TGGCCTA250.00778272750.9997945
TGTACTC250.00778272750.9997943
ACAAGGT452.9140358E-547.222033
AGATGTG901.2732926E-1147.222039
CCCTTGA651.0493022E-745.7848321
GTATCAA13400.042.514491
GCAGCGT801.1143129E-842.514491
CGTCAGA851.9084837E-839.999845
GGTATCA6800.038.763211
TATCAAC15250.037.344112
AGCGTCA955.084803E-835.789333
CAGATGT955.084803E-835.789338
TTATCAA601.5887107E-435.4165239
TTAAACG301.5926319E-435.41652322-23
Produced by FastQC (version 0.11.5)