FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145047
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5360.3695353919763939No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.2778409756837439No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG2340.16132701813894806No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.1585692913331541No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTGA1990.1371969085882507No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG1620.11168793563465634No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.10824077712741388No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA1490.1027253235158259No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTCTCTTATACACA1460.10065702841148041No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGTC150.005776040354.9937938
CACCGCG150.005776040354.993798
TATCGGG150.005776040354.9937944
GTACGGT150.005776040354.9937914
GTGGTAT5300.047.7798921
TGGTATC5600.045.220252
CGACGAA358.6009706E-539.3083955
GCCTTGA300.001911406436.7004931
CGGGGGA701.5940714E-731.42502617
TTCTCAT350.00409019631.42502633
ATACGCC801.48465915E-830.9340127
ACGGGGG1101.2732926E-1129.99661616
GGTATCA9100.028.7055513
GTATCAA19400.028.6604371
TGAGGAC400.00784195127.5063823
GGCAGAA400.00785516927.49689752
GAGACCC400.00785516927.49689736
TACGCCT904.665162E-827.49689728
AGAGTAC11500.025.82317411
CAGAGTA11750.025.5077610
Produced by FastQC (version 0.11.5)