FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125815
Sequences flagged as poor quality0
Sequence length61
%GC34

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11510.9148352740134323No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.2209593450701427No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.1931407224893693No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.14783610857210985No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGATC150.005730836755.098771
ATCATAC150.005757990355.033023
CTTGCTG150.005767063255.01113546
CTGCTAC202.6071773E-454.9892718
CAGCACA150.005776146454.989274
TAAACTC150.005776146454.989274
CCCTTTC150.005776146454.9892712
GCTACGC202.6071773E-454.9892720
CTACGCA257.837272E-443.99141321
TGCTACG257.837272E-443.99141319
TTAAACG403.9171427E-641.2419522
GTGGTAT4800.040.1761861
TGGTATC5300.036.342562
CCACTGC704.0308805E-935.35024315
ACCACTG704.0308805E-935.35024314
CTGCTTA401.8941335E-434.36829418
GTCGACA401.8941335E-434.3682948
GACATTT401.8941335E-434.36829411
TCGACAT401.8941335E-434.3682949
GTCGAGA350.004089799731.422448
Produced by FastQC (version 0.11.5)