FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147464
Sequences flagged as poor quality0
Sequence length91
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA13600.9222589920251721No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA7960.539792762979439No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.5235176042966418No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA6380.4326479683176911No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.42857917864699185No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.40416644062279605No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT4640.3146530678674117No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.29566538273748166No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3300.2237834318884609No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA3160.21428958932349593No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.18716432485216733No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA2680.1817392719579016No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2510.17021103455758693No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.1559702707101394No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC2300.1559702707101394No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA2250.15257961265122336No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.14918895459230727No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC2090.14172950686269192No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.12070742689741225No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1780.12070742689741225No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.11324797916779689No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.10714479466174796No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA1560.10578853143818152No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA1560.10578853143818152No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.10375413660283189No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG1530.10375413660283189No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA1520.10307600499104867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3850.070.645412
GTGGTAT3800.070.45661
CTCTATG200.003218622263.746441
CAATACA200.003218622263.746444
TTGCACA509.047526E-751.031769
TCACGAA354.7984542E-448.6016779
GGTTATC802.0736479E-1047.8422787
GACAGGT452.9151688E-547.2195851
GTATCAA19150.043.7181851
CCTACTA409.295506E-442.4976272
GTAGAGC209.310679E-442.49762784-85
TCGTAGA209.310679E-442.49762782-83
GGTATCA9050.041.3236581
CGACTTT1350.040.923643
AAGGTTA951.1314114E-940.260915
CAAGGTT951.1314114E-940.260914
GTCGACT1400.039.4620821
TATCAAC22350.038.0291942
CCCATGG450.00165328137.8013088
ACAAGGT1053.0140654E-936.4265373
Produced by FastQC (version 0.11.5)