FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436080
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA27850.6386442854522106No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA14920.3421390570537516No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.32562832507796735No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.32516969363419557No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA13120.30086222711429095No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.2685287103283801No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.2171619886259402No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT8950.20523757108787377No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA8090.18551641900568702No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA6940.15914511098880937No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA6140.14079985323793798No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.11786828104934874No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA4520.1036507062924234No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.10021097046413502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1400.069.836044
GTGGTAT7000.068.037631
TGGTATC8100.059.3025252
AGCGTCA1700.052.510983
GTATCAA33800.043.4041861
CGTCAGA2200.042.508895
GGTATCA16450.039.809251
TATCAAC38300.038.5132752
GTCAGAT2600.035.969066
GCAGCGT2400.035.4362681
ATCAACG42100.035.137993
CAGCGTC2550.035.007322
CAGATGT2700.034.628938
TCAACGC43150.034.1844374
CAACGCA43600.034.1241075
CGACTTT2500.032.3067553
AACGCAG46200.032.111696
GACAGGT2800.031.8926411
GTACTAG550.00443299330.9261971
AGATGTG3200.029.2181599
Produced by FastQC (version 0.11.5)