FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436080
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15620.3581911575857641No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10890.24972482113373692No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5780.13254448725004586No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCGC150.005781594754.99908442
CGCGTGA150.005781594754.99908413
GTGGTAT10450.045.0250631
TGGTATC11500.040.4216422
TAGAGCG300.001924471936.66605452
TCTACGG300.001924471936.66605446
GCGTGAC300.001924471936.66605414
TACTAGG401.897243E-434.3823132
AGAGGCG350.004097203731.4280511
ATACGCC1150.031.08643727
TATAGTA553.4808705E-530.006382
GTATCAA43450.028.8768351
TACTGGT507.0161454E-427.505852
CCTACAC606.309635E-527.49954232
TATATCG400.00786850527.4995425
TATGCTC400.00786850527.49954241
TTAAACG400.00786850527.49954222
TACGCCT1303.6379788E-1227.4995428
TATCAAC47350.026.3730832
AGAGTAC23150.025.42074411
Produced by FastQC (version 0.11.5)