FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316647
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA23750.7500465818403459No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.3808657590313504No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA11470.36223302289300074No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA11120.3511797048448272No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.34486352310301377No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.29307083282014357No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.238751669840548No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT7130.22517187909564912No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA5950.1879064068189498No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5350.1689578615935095No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA4400.13895599831989566No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.12790268027172214No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.12284973487827139No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.10453280782701242No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA3180.10042728969483368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.060.455861
TGGTATC6850.057.086972
AGCGTCA1000.046.7554743
TTCCGTC409.303798E-442.5049747
TATCGTT255.450502E-542.49826484-85
GCGTCAG1250.040.8047754
GTATCAA24350.039.462671
GGTATCA13150.037.1833951
GCAGCGT1301.6370905E-1135.9771161
TATCAAC27850.035.865962
ATATACG16500.035.8072242
CAGCGTC1352.7284841E-1134.6336822
GATATAC16750.034.2685661
CGTCAGA1501.8189894E-1234.0039835
CGCATTG17400.033.9551247
TACGCAT17700.033.6197555
TATACGC17600.033.569273
ATACGCA17750.033.5250474
GCATTGT17600.033.333038
ATCAACG30850.032.515963
Produced by FastQC (version 0.11.5)