FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316647
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11370.359074932022094No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7970.25169984241126553No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4140.13074496205553818No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATC150.005777324555.00584424
AGACGAC150.005777324555.00584421
AATACGA150.005780937654.99715852
CATCGCA150.00578455254.98847227
ATCGCAG150.00578455254.98847228
GTGGTAT8700.047.486351
TGGTATC9400.043.887642
CGAGGGA401.8972575E-434.37865410
TTAAACG501.8145316E-533.00350622
GCCTTGA453.7555752E-430.6023121
GAGAGTT653.1973268E-629.61385230
GGTAGAC400.00780618427.5420821
AAGCCGT400.00786058327.50292211
AACGCTT400.00786058327.50292218
GATGCTC400.00786058327.50292222
GTATCAA34650.027.3433651
GACAGGT858.643583E-725.921961
AGAGTTG858.7636727E-725.88101631
GGTATCA16950.025.3084283
GGCTAGC550.001213720225.0382581
Produced by FastQC (version 0.11.5)