FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446381
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA25600.5735011122785244No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.3290910679442002No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.2874226277552136No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA12190.2730850999482505No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA11720.26255597796501196No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.2390334714067131No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.19714100734574275No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT8000.17921909758703888No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.13329420383036014No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA5180.11604436568760768No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA5120.11470022245570488No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.1086515779121423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6100.053.652561
TGGTATC7250.046.314682
GCGTCAG1800.044.865314
CGTCAGA1950.043.5938225
GTATCAA30050.043.4233671
GGTATCA14300.042.801211
TATCAAC34950.037.821852
AGCGTCA2150.037.5616573
ATCAACG38800.034.178453
GTCAGAT2400.033.6489836
GCAGCGT2400.033.6489831
TCAACGC40500.032.74384
GTAATAG652.5494638E-432.6953661
TAGGGCA652.5494638E-432.6953665
CAACGCA40700.032.4784665
ATATACG17950.031.9667822
ATACGCA18600.031.3066944
CGCATTG18300.030.8874057
AACGCAG43150.030.831396
TATACGC18700.030.684693
Produced by FastQC (version 0.11.5)