FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446381
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18330.41063575734630287No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9410.21080646353675447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9300.048.5317881
TGGTATC10050.045.1585432
GTAACCA350.004097340631.42799453
ATACGCC453.7922693E-430.55499527
GTATCAA39100.029.1399631
TTAAACG606.310035E-527.49949622
GTTATTC507.025889E-427.4994963
GGTGCTA400.00786876727.49949511
TATCAAC43800.026.2496932
AGAGTAC21400.025.70046211
CAGAGTA22100.024.88642310
GGTATCA18800.024.5740183
CGAGGGG4050.024.44399610
CGGGAAA1700.024.26426117
ATCAACG47850.023.8501383
TCTATAC701.7984454E-423.5709953
TCAACGC48650.023.4014234
CAACGCA49250.023.1163275
TACGGGT1800.022.91624615
ACGGGTA1202.9904186E-822.91624616
Produced by FastQC (version 0.11.5)