FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761754
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19710.2587449491568144No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.1056771608682068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10500.044.578131
TGGTATC11600.040.5456162
ATACACG606.316246E-527.4992124
GTATCAA42250.026.4582751
TTAAACG957.96681E-826.05188622
GTACTAG550.001217489125.03051
GGTATCA21000.024.6490841
TATCAAC45700.024.1944452
AGAGTAC24100.023.9619711
CGAGGGG4050.023.76475110
TACACGG701.8001969E-423.5707535
CAGAGTA25400.022.952110
TACGCCT600.002035242522.9160128
ATCAACG49600.021.8441313
CAACGCA50100.021.7907925
TCAACGC50100.021.7907924
AACGCAG51850.021.2144366
TCGAGGG14750.019.7621469
TATGGGT700.00497534819.6422944
TAAACGC1401.5289152E-719.64229423
Produced by FastQC (version 0.11.5)