FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329016
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA196120.5891230321512423No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT112730.33862859175203724No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99800.2997882857877523No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA96560.29005568011688737No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA81170.24382580318027908No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77130.23169008499808955No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT73680.2213266622930019No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73450.22063576744599606No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA51200.15379920072477873No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT43030.12925741420287556No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT42020.12622348465732816No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA37360.11222535427886197No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA35280.10597726174941785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT59850.061.9341431
TGGTATC61000.060.968262
AGCGTCA11950.043.3927843
GCGTCAG13450.039.502074
GGTATCA119050.038.7416531
GTATCAA225750.038.3190271
CGTCAGA16250.033.7428325
TATCAAC261600.033.5349432
CAGCGTC16700.032.0685772
GCAGCGT16850.031.5346431
ATATACG144150.031.1663532
GATATAC139750.030.8131051
ATCAACG293400.030.175523
CGCATTG148900.029.9428657
TCAACGC296500.029.7887974
ATACGCA151650.029.681494
TCAGATG18200.029.6582097
CAACGCA298700.029.5845135
TATACGC152350.029.4051743
TACGCAT154600.029.0335165
Produced by FastQC (version 0.11.5)