FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206612
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA194700.6071829083156927No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT116590.3635924770443072No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109670.342012067565393No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA100790.31431928777164186No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84320.2629566657893128No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT79570.2481435234446824No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76950.23997290598301257No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA72640.22653192840293743No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT55430.17286157477112915No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT43300.1350334870573677No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA39300.12255926192504737No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA35550.11086467586349705No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA34580.10783967626890936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT60950.062.8361131
TGGTATC62000.062.1060332
AGCGTCA6200.058.267253
CAGCGTC6900.052.356082
GCAGCGT7100.049.691031
GCGTCAG7700.048.5723654
CGTCAGA7750.048.2597475
GGTATCA130700.040.2302131
GTATCAA259000.039.930121
ATATACG143000.034.6842352
TATCAAC302950.034.4692272
GATATAC142050.034.382471
ATACGCA148300.033.501994
TATACGC151550.032.783543
CGCATTG152900.032.46737
GCATTGT151250.032.3999828
CAGTGGT10700.031.7807731
TACGCAT157100.031.7611335
ATCAACG341950.030.6373793
CAACGCA344900.030.4620675
Produced by FastQC (version 0.11.5)