FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206612
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166010.5177115285541251No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65000.20270615840020556No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36710.11448220115186994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT83450.046.83421
TGGTATC85250.045.5894472
GTCGTAT501.8104674E-533.028011
CGTACGG453.7979562E-430.5543114
TGCGACG1950.029.61509922
GCGACGA2100.028.80924623
TACTAGG2300.028.7011932
GTACTAG2400.028.6701471
GCGTACG507.035722E-427.49930813
CGTAAGT400.00787617227.49930650
GTATCAA328750.026.5312441
TCGTATC651.08813016E-425.3895192
GCATCGT2600.025.3839768
GGTATCA167700.024.7824951
TCGCACA1001.311746E-724.7489939
ATCGTCA2900.024.65454910
ATACGCC6400.024.49080727
TGCGCGA801.609713E-524.06189212
AGAGTAC173250.023.7930511
GACGTTC701.8019105E-423.5708338
Produced by FastQC (version 0.11.5)