FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509528
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96520.2750227381003941No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47950.13662805938576356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT68250.046.938721
TGGTATC73500.043.6715132
TGCGACG2100.024.88033522
GTATCAA245850.024.8745231
GCGACGA2000.024.74938423
GGTATCA133300.024.5076391
CGACGAG2050.024.1457424
AGAGTAC123800.022.63441311
CAGAGTA129600.021.9185110
TATCAAC284950.021.5157322
CGAGGGG26300.019.76158310
TATGTCG700.00497792919.6429345
ATCAACG312250.019.5431843
CAACGCA311100.019.3670855
TCAACGC313750.019.27394
AACGCAG319100.019.0194266
GTACTAG4800.018.9214151
AGTACGG147350.018.69964213
TACGGGC50650.018.5678815
CGGGCCA45500.018.55448317
Produced by FastQC (version 0.11.5)