FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156834
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107410.34024595528304624No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53470.16937856092528147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT72200.046.8061071
TGGTATC76850.043.7011572
TGCGACG2400.027.50037222
GTATCAA268450.025.863971
CGCTTAC651.08980494E-425.38375327
GGTATCA142100.024.4015731
AGAGTAC138300.023.204211
CCGAATT4350.022.75784914
TATCAAC310050.022.4090122
CGACGAG2600.022.2118424
CAGAGTA145600.022.21078310
TCTAGCG650.00324162721.15312850
ATCAACG333650.020.5737133
GCTCGTC3500.020.42787739
TCAACGC337900.020.2072164
CAACGCA340050.020.063285
ACGAGTC2900.019.91343326
GTACTAG4150.019.8936521
CTAGTAC4850.019.8465733
AGTACGG156900.019.78740913
Produced by FastQC (version 0.11.5)