FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860497
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA81010.4354212879676775No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71360.3835534268531473No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT67100.3606563192523288No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA53500.2875575719821102No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.27659275989157733No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.2663804349052968No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT43960.2362809507352068No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA40860.2196187362839069No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA30820.1656546610932455No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.14329504428117862No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA26310.141413826520548No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25360.1363076640274077No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA24040.1292127856158865No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA23380.1256653464101259No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.10534819459531514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT36700.057.2180861
TGGTATC39450.053.7610322
GCGTCAG7650.048.8917164
AGCGTCA8100.044.6013453
GTATCAA146550.040.8113251
CGTCAGA9250.040.4380345
GCAGCGT9650.037.442431
GGTATCA75750.036.812361
TATCAAC171400.035.3112642
TCAGATG10850.035.258347
ATCAACG191400.031.6880933
GTCAGAT12000.031.17356
CAACGCA194350.031.122145
TCAACGC195300.031.0117784
CAGCGTC11950.030.5875432
AACGCAG202800.029.995486
CAGATGT13250.028.5511238
GTACTAG3950.027.980061
GTCGACT14350.026.95641
AGATGTG14650.025.8226879
Produced by FastQC (version 0.11.5)