FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860497
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44240.23778592494371129No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28900.15533483794921465No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA21870.11754923549997662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT50000.045.9489141
TGGTATC51200.044.3214872
TTACGCA501.820141E-533.001213
ATCGACG507.03206E-427.5010093
ATGCGAC1203.6379788E-1127.49952921
GTATCAA186950.026.5356061
TACGCGG651.08904664E-425.38554853
TGCGACG1301.0004442E-1025.38418222
TCGACGC651.0896434E-425.3835014
GGTATCA95900.024.4899483
AGAGTAC103050.023.90967411
CAGAGTA106600.023.47457710
TATCAAC212900.023.2555792
TATCGGC952.312745E-623.1631012
GTATCGG852.561104E-522.6588521
CGAGGGG20750.022.13155710
GTATCGA752.8634854E-422.0114561
TCGTCTA1757.2759576E-1222.00139854
GAGGGGT18700.021.32259211
ATCAACG234900.021.0267413
Produced by FastQC (version 0.11.5)