FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130399
Sequences flagged as poor quality0
Sequence length61
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA2340.17944922890512963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.17868235185852654No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGAAGAAGCTTCAT2220.17024670434589223No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.14724039294779867No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAA1880.1441728847613862No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTATTAATTCGGGGC1780.13650411429535503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1610.12346720450310202No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACG1610.12346720450310202No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTCCATTTTCGTCT1560.11963281927008644No Hit
CTTCTAAGTCTTCATATTTTCTCTTAAAATCTTAAGCTATTAAAATTACGTTAAAAACTTA1530.11733218813027707No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAAC1450.11119717175745213No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA1420.10889654061764277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC150.005767213655.01189817
TCTTGGG150.005775979754.99079512
TGGGTAG150.005775979754.99079515
GGGTAGC150.005775979754.99079516
TCGATTA150.005775979754.99079513
GTTCGAT150.005775979754.99079511
TGCCGCC150.005775979754.99079550
TTGGTGT303.469648E-545.8432554
GTGGTAT2850.045.3780981
GGTAGCA257.822445E-444.00951417
CTACGGG257.8372494E-443.99263414
TGGTATC2900.043.6301232
CAGTGGT358.595006E-539.3092961
CGATTAC300.001920952336.6605314
AAGGCTT558.6327964E-734.9941431
GCAGTAG401.8942001E-434.36924716
GTGTATA501.8002902E-533.019811
GGCTTAT601.7040311E-632.07796533
ATTAGTC350.004082104731.4353718
GTAGCAG350.004082104731.4353718
Produced by FastQC (version 0.11.5)