FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572598
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA102260.6502615417290369No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.363347785002906No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA54680.34770488071331646No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52770.3355593737242449No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA45440.2889486060646141No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.2711436743528861No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT38130.24246501648863852No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.23934915343908617No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA25410.16157975528393143No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.13881487830965067No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA20650.13131137137399385No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA19580.12450734389844068No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.10206041213329789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT32100.057.474971
TGGTATC32250.057.1967282
CGTCAGA5050.042.924365
GCGTCAG5200.041.6848264
AGCGTCA5500.040.183883
GGTATCA65150.039.998171
GTATCAA123000.039.676271
TATCAAC143850.034.2735672
TCAGATG6850.031.6429527
ATCAACG161350.030.714313
CAACGCA164550.030.2471185
TCAACGC163950.030.2013034
GTCAGAT7400.029.8673466
GCAGCGT7150.029.7274741
CCTTGAT10050.029.6035082
ATATACG82650.029.3118482
GATATAC80350.028.9397961
AACGCAG173200.028.9328296
ATACGCA84650.028.7699344
CGCATTG84750.028.3840337
Produced by FastQC (version 0.11.5)