FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214601
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA90550.7455123122737426No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.49456570511633036No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.43858024157727515No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.3852293880871167No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA43880.36127090295496217No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA43400.3573189878816171No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39130.32216341004165155No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT35970.29614663580879647No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA25530.2101924829635411No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.1770128626602481No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20320.16729773810494147No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19570.16112287080283977No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA19510.16062888141867165No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA18080.14885546776266445No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA15850.13049552898441547No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC15150.12473231950245389No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.11147693769394229No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.1085130013889335No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA13020.10719569636448512No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC12950.10661937541628896No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC12840.10571372821198072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28050.061.9873471
TGGTATC33600.052.112922
GTATCAA120000.041.413881
GCGTCAG3800.039.1446044
GGTATCA59900.038.7506031
AGCGTCA4000.036.1248783
TATCAAC139900.035.8165322
GACCTTA2100.034.4046447
ATCAACG154950.032.7765923
TCAACGC158400.032.0090454
CAACGCA161500.031.4998935
GAATAAG6650.031.324711
ATATACG71200.030.6213842
ATACGCA71650.030.4883824
GATATAC69250.030.4491061
CGCATTG71600.030.390967
AACGCAG168000.030.3064426
TACGCAT72700.030.0480425
CCCTTGA5250.029.960911
ACGCATT74550.029.5304186
Produced by FastQC (version 0.11.5)