FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214601
Sequences flagged as poor quality0
Sequence length61
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33300.2741641082133145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33210.27342312413706227No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA18330.15091375686336503No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18020.1483614783784963No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG13470.11090061674574614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT36850.044.5144961
TGGTATC40650.040.27062
GTATCAA151500.027.7044731
CGAACTA400.00787468827.49901229
TCTCGCG606.319602E-527.49901227
TTAAACG1400.027.49901222
GGTATCA75700.025.704921
TCGCGCC759.755744E-625.66574729
CGAATTA2250.024.44356715
TATCAAC173950.024.0883562
AGAGTAC85250.023.38625711
CGAGGGG19200.023.05906710
CAGAGTA87400.022.87389410
GTCCAAT852.5540643E-522.665841
GCCCCGA2500.021.9992111
CCCCGAA2250.021.9992112
ATCAACG192650.021.6305713
TCAACGC194350.021.2839344
CAACGCA196000.021.1731765
TCGACTC1055.5199616E-620.9516289
Produced by FastQC (version 0.11.5)