FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116184
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA48500.43451617296073053No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.30801373250288483No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34060.30514682167097895No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA28990.2597242031779706No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA25570.22908409366197688No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.22245436236319457No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.2154662672104241No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT23450.21009080940060065No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.12462103022440743No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA13350.1196039362685722No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA11930.10688201945199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20450.055.505591
TGGTATC22600.050.021392
GGTATCA44550.040.2701721
GTATCAA81400.039.379091
GCAGCGT3700.036.7677421
TATCAAC94900.034.0801052
ATCAACG102800.031.5851383
TCAACGC104350.031.1567024
AGCGTCA4250.030.9995423
CGTCAGA4400.030.910025
CAACGCA105900.030.7421915
GCGTCAG4300.030.6390824
GCCGATA703.9435094E-430.3566952
AACGCAG111200.029.4324786
GATATAC42900.026.7562471
ATATACG43650.026.6777292
ATACGCA44950.026.0007294
CGCATTG45100.025.7292447
TACGCAT46300.025.610925
CGCAGAG127750.025.519698
Produced by FastQC (version 0.11.5)