FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116184
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33050.29609813435777615No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17130.15346932047046008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27100.044.8984071
TGGTATC28900.041.603312
ACGTCGT350.00410059731.42698748
TAACGTT453.7946078E-430.55675154
AGAACGT400.00787497927.49861145
GTTCGCC606.3197804E-527.4986111
GTATCAA104100.026.5497781
GGTATCA53100.025.1953091
AGAGTAC57450.023.78905111
TATCAAC117850.023.4161572
GAACGGG600.002036091622.91550814
CAGAGTA60200.022.88505610
GACCTTA2150.021.7430887
ATCAACG128750.021.3444373
TCAACGC129250.021.2329714
CAACGCA130500.021.0295895
TGCCGAC3700.020.81162555
AACGCAG133900.020.7420446
AGTACGG66150.020.41091213
CGGGCCA23350.020.37370317
Produced by FastQC (version 0.11.5)