FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035624
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA41330.3990830648961399No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT32270.3115995766803396No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.26544382903447583No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA22850.2206399233698717No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.21079078893498027No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT21100.20374189860412661No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.19398932431075372No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA18920.18269178775308414No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA12130.11712745166199316No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.11075448232176928No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA10790.10418839269850835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17350.060.0428161
TGGTATC19600.053.774992
AGCGTCA3500.049.7852333
GCGTCAG3800.045.8548244
GTATCAA64250.038.317741
CGTCAGA4650.037.4727555
GGTATCA35850.034.988661
TATCAAC73250.033.941652
GCAGCGT5650.033.1130141
ATCAACG81000.030.9564913
CAGCGTC5650.030.8404122
TCAACGC83200.030.2400914
CAACGCA83500.030.233245
AACGCAG86900.029.2459776
TACCGAC755.9039175E-428.3344297
TCGAGGG21250.027.6010679
GATATAC35700.025.6073531
ATATACG37250.025.55682
TCAGATG6850.025.4389387
ACGTCGG850.001226541624.999766
Produced by FastQC (version 0.11.5)