FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035624
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25090.24226939507002543No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16520.15951735378863371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22250.049.2363551
TGGTATC24150.044.9881442
GGTATCA42150.026.0986233
GTATCAA81450.026.0553021
AGAGTAC45000.024.3211311
CAGAGTA46650.023.63774110
TATCAAC92050.023.097972
ATCCGCT752.873262E-421.9990168
ATCAACG98800.021.1549423
ACGGGCC21950.020.92161416
TCAACGC99700.020.7688794
CGGGCCA21850.020.76566117
CAACGCA100150.020.7030185
AGTACGG51750.020.67057213
TATACCG804.4432093E-420.6240755
TTAAACG1751.8553692E-1020.42864222
TACGGGC23950.020.3226815
GAGTACG53700.020.0735912
AACGCAG104250.019.967936
AGATATA22950.019.65148423
Produced by FastQC (version 0.11.5)