FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44451
Sequences flagged as poor quality0
Sequence length91
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA4481.0078513419270658No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.9988526692312883No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA3270.7356414928798003No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.7221434838361341No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.6254077523565273No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.5916627297473622No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT2020.4544329711367573No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA2000.4499336347888686No Hit
ATTGATAACCTTGTTTTGCACTAAAAGCAAAAAAAAAAAAAAAAAAAAAA2000.4499336347888686No Hit
ATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAA1980.4454342984409799No Hit
GTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAA1970.44318463026703564No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.4004409349620931No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.3284515533958741No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.30595487165643065No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.27445951722120987No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA1210.27220984904726553No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA1170.26321117635148816No Hit
AAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATACCTGTCTCT1130.2542125036557108No Hit
GAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAA1100.24746349913387775No Hit
ATTGATAACCTTGTTTTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.24296416278598906No Hit
GGTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTA1060.23846482643810038No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA1030.23171582191626733No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.22721648556837865No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.21146880835076826No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.20921914017682391No Hit
TATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAA850.19122179478526916No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.18897212661132481No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA820.18447279026343613No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.18447279026343613No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.18447279026343613No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA820.18447279026343613No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT810.1822231220894918No Hit
ACCTTGTTTTGCACTAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1777237857416031No Hit
GTATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTCTGTCTCT730.16422577669793706No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAA650.1462284313063823No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.14397876313243796No Hit
ATTATTGATAACCTTGTTTTGCACTAAAAGCAAAAAAAAAAAAAAAAAAA630.14172909495849362No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.1349800904366606No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG600.1349800904366606No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAG570.12823108591482757No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12598141774088323No Hit
TTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12598141774088323No Hit
GTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAA540.12148208139299453No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA530.11923241321905019No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.11698274504510583No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA520.11698274504510583No Hit
AAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATACCCTGTCTC510.1147330768711615No Hit
GTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA510.1147330768711615No Hit
ATTATTGATAACCTTGTTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.11023374052327281No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA490.11023374052327281No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.11023374052327281No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.11023374052327281No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.10348473600143979No Hit
GTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.10123506782749545No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT450.10123506782749545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1450.058.620692
GTGGTAT1550.054.8387071
AGACAGG803.6379788E-1253.1258
GACAGGT651.7316779E-952.307699
ATAAGAG1300.042.53
AGAGACA1300.042.56
TAAGAGA1350.040.9259264
GAGACAG1350.040.9259267
ACTTTTT1056.366463E-1140.476191
GTGTATA851.8577339E-840.01
TGTATAA851.8577339E-840.02
GGTATCA4300.038.546511
GTATCAA8100.037.777781
ACAGGTA453.13521E-837.7777810-11
TATAAGA1500.036.8333322
AAGAGAC1500.036.8333325
CAAGGTT1052.912202E-936.428574
GGTTATC1104.5893103E-934.7727247
GTTATCA1104.5893103E-934.7727248
CAATGCG500.002760381434.05
Produced by FastQC (version 0.11.5)