FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38630
Sequences flagged as poor quality0
Sequence length91
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA5831.5091897488998187No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3941.01993269479679No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.8309603934765726No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA2980.7714211752523945No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA2960.7662438519285529No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.595392182241781No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.49702303908879103No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.4659590991457417No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1720.4452498058503754No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1700.4400724825265338No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1680.4348951592026922No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA1320.34170333937354386No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA1230.3184053844162568No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.315816722754336No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC1170.3028734144447321No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA1140.2951074294589697No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA1140.2951074294589697No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.2847527828112866No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.26922081283976185No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC1010.2614548278539995No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.2226249029251877No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.2200362412632669No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.2200362412632669No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA820.21227025627750454No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG810.20968159461558375No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC800.207092932953663No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA800.207092932953663No Hit
TTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATAC690.1786176546725343No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA670.17344033134869272No Hit
GAATAAGCTGGTATCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA660.17085166968677193No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.16826300802485114No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.16567434636293035No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTG630.16308568470100956No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT630.16308568470100956No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA610.157908361377168No Hit
GATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCT600.1553196997152472No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.1553196997152472No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA600.1553196997152472No Hit
GATATACGCATTGTGGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.1553196997152472No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA600.1553196997152472No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.15014237639140562No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGCTGTCTCT580.15014237639140562No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.14496505306756405No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG520.13461040641988092No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.13461040641988092No Hit
TTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.13202174475796014No Hit
GATATACGCATTGTGGCAGTACATCAAGGGCTGTCTCTTATACACATCTC500.12943308309603935No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTT500.12943308309603935No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA480.12425575977219776No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.12425575977219776No Hit
GTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA470.12166709811027698No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCCTGTCTCTTATACACA470.12166709811027698No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.12166709811027698No Hit
TACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAA470.12166709811027698No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1190784364483562No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG450.11648977478643542No Hit
TATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAA450.11648977478643542No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTG450.11648977478643542No Hit
ATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGG450.11648977478643542No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG440.11390111312451463No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACAT440.11390111312451463No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT420.10872378980067306No Hit
ATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAA420.10872378980067306No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATGTCGTATGCCGT410.10613512813875227No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTT410.10613512813875227No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGT400.1035464664768315No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1035464664768315No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGT390.1009578048149107No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAC259.0417685E-567.998964
GGACTTT200.00320011763.749037
ACTTTCC200.00320011763.749039
TGGGACT200.00320011763.749035
CTGGTAT508.912248E-750.999228
GGGACTT250.00774009950.999226
GACTTTC250.00774009950.999228
CCCTTGA354.7387433E-448.6336821
GAATAAG452.8523178E-547.282751
GTATCAA9100.047.230791
GCTGGTA551.7191451E-646.362937
AAGCTGG551.7191451E-646.362935
AGCTGGT551.7191451E-646.362936
GGTATCA4000.045.746061
ATAAGCT603.1290328E-642.499353
TAAGCTG603.1290328E-642.499354
GGGTACC209.266085E-442.4993518-19
TTTGGGA409.208406E-442.499353
TATCAAC10650.041.9007722
GTGGTAT1450.041.087081
Produced by FastQC (version 0.11.5)