EGAF00005411803 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005411803
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38630
Sequences flagged as poor quality	0
Sequence length	61
%GC	34

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1310	3.391146777116231	No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	176	0.45560445249805853	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG	138	0.3572353093450686	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTGA	102	0.26404348951592027	No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGA	87	0.22521356458710845	No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG	87	0.22521356458710845	No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA	83	0.21485891793942533	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTCTCTTATACACA	82	0.21227025627750454	No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.21227025627750454	No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCCTGTCTCTTATACACATCTGACGCTGC	60	0.1553196997152472	No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59	0.1527310380533264	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTGTCTCTTATACA	56	0.14496505306756405	No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA	55	0.1423763914056433	No Hit
CTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT	53	0.1371990680818017	No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCC	49	0.12684442143411856	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCCTGTCTCTTATAC	47	0.12166709811027698	No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTG	46	0.1190784364483562	No Hit
CCCTTGATGTACTGCCACAATGCGTATATCCTGTCTCTTATACACATCTGACGCTGCCGAC	44	0.11390111312451463	No Hit
GAATAAGCTGGTATCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC	43	0.11131245146259384	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGCTGTCTCTTA	41	0.10613512813875227	No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGCTGTCTCTTATACACATCT	39	0.1009578048149107	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTAC	15	0.005742045	54.998707	22
GTGGTAT	205	0.0	47.01101	1
TGGTATC	295	0.0	33.558533	2
GTCATGT	35	0.004025961	31.468592	1
ATTGGGC	35	0.004051651	31.427828	8
TCATGTA	35	0.004051651	31.427828	2
TGGGCAA	35	0.004051651	31.427828	10
ATGTATT	35	0.004051651	31.427828	4
AATAAGC	35	0.004051651	31.427828	2
AAGCTGT	35	0.004051651	31.427828	49
CATGTAT	35	0.004051651	31.427828	3
TCGACTT	45	3.7291515E-4	30.554836	2
CGACTTT	45	3.7291515E-4	30.554836	3
GTATCAA	980	0.0	30.344715	1
GAATAAG	40	0.0077325064	27.535019	1
GTATTGG	40	0.007781696	27.499353	6
TATTGGG	40	0.007781696	27.499353	7
TTGGGCA	40	0.007781696	27.499353	9
CGCCTGT	50	6.909692E-4	27.499352	30
TACGCCT	50	6.909692E-4	27.499352	28

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content