FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180043
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5510.3060380020328477No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4920.2732680526318713No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.16551601561849114No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.1438545236415745No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG2200.12219303166465789No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACC150.005777450354.99583452
GTGGTAT4900.044.4073871
CAATCAC257.841701E-443.99666637
TGGTATC5300.041.5062872
GTACTAG300.001906347536.7250981
ATAAACG601.7075963E-632.0809023
CAGCATT350.004058764331.4786551
AACAATC350.00408649131.43492535
TATACGA350.00409205531.42619127
CAGTGGT1101.0913936E-1130.0478061
GTATAAA1052.2191671E-1028.8554341
AGTGGTA1052.2737368E-1028.807342
GTATCAA22800.028.5102751
GCCTTAG400.00785870227.497926
CTCTTAA400.00785870227.4979229
GTACTGA400.00785870227.497926
AAGATTG400.00785870227.4979210
GGTTATC507.0118514E-427.4979177
TATCAAC25750.025.308762
GGTATCA10600.025.2051941
Produced by FastQC (version 0.11.5)