FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136136
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA11080.8138919903625786No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.5237409649174355No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA5680.41722982899453487No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.4164952694364459No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA5470.40180407827466647No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.37389081506728566No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT4150.3048422166069225No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.2806017511899865No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA2920.2144913909619792No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.21008403361344538No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.17115237703473No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA2290.1682141388023741No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA2290.1682141388023741No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.14544279250161604No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.13956631603690428No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.11899864841041312No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.11532585061996828No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC1490.1094493741552565No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.10651113592290062No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1430.10504201680672269No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.10063465945818886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA401.9439358E-763.753
GTGGTAT3650.062.8767131
GTCAGAT501.32986315E-859.56
TGGTATC4100.054.9390262
TCAGATG552.8212526E-854.0909047
CGTCAGA552.8212526E-854.0909045
GCGTCAG509.0812864E-751.04
TATGTAG250.0077815851.02
AGCGCAG409.2904264E-442.55
GCAGCGT505.4265824E-542.51
CAGCGTC603.1879663E-642.4999962
GTATCAA14100.041.8971631
ATAAGCT753.2355456E-739.6666683
GACAGGT655.525839E-639.230771
CAAGGTT450.001657899537.777784
ACAAGGT450.001657899537.777783
CTTACAC450.001657899537.777783
TATCAAC16000.036.656252
AATAAGC709.188434E-636.428572
GGTATCA7100.035.9154931
Produced by FastQC (version 0.11.5)