FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110090
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA10180.9246979743845943No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.5150331546916159No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA5230.47506585520937417No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.47234081206285766No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA4600.4178399491325279No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.39603960396039606No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT3610.3279135252974839No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.3251884821509674No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.21164501771278044No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA2080.18893632482514308No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA1910.17349441366154963No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.1635025887909892No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.14442728676537378No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA1590.14442728676537378No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.12898537560178036No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA1370.12444363702425289No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1340.1217185938777364No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC1330.12081024616223089No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.11263511672268145No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.10718503042964847No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1140.1035516395676265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3250.066.722271
TGGTATC3600.061.388562
GCAGCGT454.366957E-756.692121
AGCGTCA509.0655885E-750.999733
GCGTCAG551.7486218E-646.3633884
AATTAGG409.26214E-442.519091
ACTTAAT209.3032117E-442.4997720-21
GTATCAA12650.042.014911
CAGCGTC655.5163873E-639.2305562
GTCAGAT655.5163873E-639.2305566
TATCAAC14100.038.2799382
ATTAGGG450.001656563337.7775762
ACAGGTA450.001656563337.7775762
GGTATCA6300.035.7537773
TTAATGG301.5913976E-435.41647722-23
AGATGTG601.5862356E-435.4164779
ATCAACG15800.034.161213
ATGGACG250.002784186733.9998226-27
TCAACGC16100.033.5246664
CAACGCA16150.033.4208765
Produced by FastQC (version 0.11.5)