FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38550
Sequences flagged as poor quality0
Sequence length91
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA8722.2619974059662775No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4941.2814526588845654No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA4451.1543450064850844No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA4251.1024643320363166No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.9416342412451363No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.8171206225680935No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.7159533073929961No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA2750.7133592736705577No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.6536964980544747No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA2440.6329442282749675No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT2340.6070038910505836No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.5473411154345007No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA1810.4695201037613489No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.3865110246433203No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.32166018158236054No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.3164721141374838No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.3138780804150454No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1120.29053177691309984No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA1110.2879377431906615No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG1010.2619974059662776No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.25421530479896237No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT980.25421530479896237No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC970.251621271076524No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA930.2412451361867704No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.23865110246433205No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA920.23865110246433205No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA910.23605706874189367No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAA880.22827496757457846No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.22568093385214008No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA870.22568093385214008No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAA870.22568093385214008No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.22308690012970167No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTG790.20492866407263297No Hit
TTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATAC780.20233463035019456No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT720.1867704280155642No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGCTGTCTCT720.1867704280155642No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.1867704280155642No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG700.1815823605706874No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.17120622568093385No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTG660.17120622568093385No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.17120622568093385No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCCT630.16342412451361868No Hit
GTGGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACAT620.16083009079118027No Hit
GAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA620.16083009079118027No Hit
GATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCT610.1582360570687419No Hit
GAATAAGCTGGTATCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA610.1582360570687419No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA580.1504539559014267No Hit
ATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGG580.1504539559014267No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA580.1504539559014267No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.14785992217898833No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG560.14526588845654992No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC560.14526588845654992No Hit
GTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAA560.14526588845654992No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC550.14267185473411154No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAA550.14267185473411154No Hit
TTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.13748378728923477No Hit
ATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAA530.13748378728923477No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTT510.13229571984435798No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCCTGTCTCTTATACACA500.12970168612191957No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.12710765239948119No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG490.12710765239948119No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAG470.12191958495460442No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.12191958495460442No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.11673151750972763No Hit
ATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAA450.11673151750972763No Hit
GCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA440.11413748378728923No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.11413748378728923No Hit
GTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.11154345006485084No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACAT430.11154345006485084No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTT420.10894941634241245No Hit
GGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.10376134889753567No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT400.10376134889753567No Hit
CAGTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTT400.10376134889753567No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.10116731517509728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1800.073.194441
CAGATCA200.003199892163.751
CCCTTGA200.003199892163.751
TGGTATC2250.058.5555532
GTATCAA9350.046.3636361
TTAAACG209.265315E-442.522-23
TATCAAC10800.041.7129632
GGTATCA4000.037.18751
ACGGGTA301.5817834E-435.41666416-17
GGTAGAT301.5817834E-435.41666418-19
AAGCTGG601.5671448E-435.4166645
AGCTGGT601.5671448E-435.4166646
CGTCTTC301.5817834E-435.41666484-85
ATCAACG12950.035.1158263
TCAACGC13000.034.980774
CAACGCA13150.034.904945
AACGCAG13400.034.253736
CACTGCT250.00277295834.016-17
AAACGCC250.00277295834.080-81
AAACGCA250.00277295834.024-25
Produced by FastQC (version 0.11.5)