FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38550
Sequences flagged as poor quality0
Sequence length61
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4591.1906614785992218No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.7963683527885863No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.5421530479896239No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.45136186770428016No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.26718547341115434No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTGA1010.2619974059662776No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG900.23346303501945526No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAA850.2204928664072633No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA840.2178988326848249No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG760.19714656290531776No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTGTCTCTTATACA710.18417639429312582No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGA660.17120622568093385No Hit
CTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT570.14785992217898833No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCCTGTCTCTTATACACATCTGACGCTGC570.14785992217898833No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA550.14267185473411154No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCCTGTCTCTTATAC540.14007782101167315No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACA540.14007782101167315No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTCTCTTATACACA510.13229571984435798No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGCTGTCTCTTA510.13229571984435798No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA510.13229571984435798No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.12970168612191957No Hit
GATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATAC500.12970168612191957No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTG460.11932555123216602No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAA460.11932555123216602No Hit
GAATAAGCTGGTATCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC450.11673151750972763No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.11673151750972763No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA420.10894941634241245No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC400.10376134889753567No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCT390.10116731517509728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)