FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31901
Sequences flagged as poor quality0
Sequence length61
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3961.2413403968527632No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.34481677690354534No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA970.30406570326948995No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.19435127425472556No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA340.10657973104291402No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.1034450330710636No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT150.00573238655.017
ACCACTG202.575186E-455.014
GTATCAC150.00573238655.04
CACTGCT150.00573238655.016
TGCTTAA150.00573238655.019
ATCACCG150.00573238655.06
CTGCTTA150.00573238655.018
TTGTGGG150.00573238655.051
ATATCAA150.00573238655.02
TACCACT202.575186E-455.013
GTGGTAT1700.046.9117661
TGGTATC2000.039.8752
ATACCAC300.001897729836.66666812
GCTGCCG601.6490358E-632.08333255
TGATAAC400.007760829327.53
GTATCAA7250.026.9310361
GGTATCA3550.026.338031
CGAGGGG1056.712071E-926.19047710
CGCTGCC759.370593E-625.66666854
AACCTTG550.001200601125.07
Produced by FastQC (version 0.11.5)