FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78673
Sequences flagged as poor quality0
Sequence length91
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA5450.6927408386612942No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.42581317605785973No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA3280.41691558730441197No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA3130.3978493256898809No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.32158427923175675No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.30251801761722574No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.25167465331180966No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1860.2364216440201848No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC1600.20337345722166436No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT1570.19956020489875814No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA1390.1766806909613209No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.1728674386384147No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA1240.15761442934678987No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA1220.1550722611315191No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC1090.13854816773225884No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA1060.13473491540935265No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.12965057897881102No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA950.12075299022536322No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.1143975696871862No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.11058431736428No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA860.10931323325664459No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC830.10549998093373839No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG820.10422889682610299No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACAGT203.0110137E-585.06
GGCTTGA200.003212324863.757
AGCTGGT200.003212324863.756
CTATTCT200.003212324863.754
CCTTGAT454.3643558E-756.666672
GTGGTAT1600.053.1251
TGGTATC1600.053.1252
AACAACG250.007769435651.01
GACAGCC250.007769435651.01
GCACCTC250.007769435651.07
ATAAGCT250.007769435651.03
CTCTATT250.007769435651.02
GGTATCA3700.047.0945931
GTATCAA8700.046.4080471
GCCTTGA409.266456E-442.51
AAGCTGG409.266456E-442.55
CTTGATG753.2123353E-739.6666643
TGATGTA753.2123353E-739.6666645
ACAGTGC559.480857E-538.636368
TATCAAC10550.038.270142
Produced by FastQC (version 0.11.5)