FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209882
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA12670.6036725398080827No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.44834716650308265No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.33971469682964717No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT6390.3044567900058128No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA6260.2982628334016257No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA5840.2782515889880981No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.23679972556007658No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT4540.21631202294622692No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA3790.1805776579220705No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.1639016209107975No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA2650.12626142308535274No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.12244975748277605No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.11863809188019936No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA2460.11720871727923309No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.11673225907891101No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10577372047150303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3900.058.8461531
AGCGTCA857.2759576E-1250.0000043
GCAGCGT857.2759576E-1250.0000041
TGGTATC4700.048.8297842
CAGCGTC901.2732926E-1147.222222
GACAGGT1051.8189894E-1244.523811
GTATCAA17850.043.809521
GCGTCAG1004.0017767E-1142.54
CGTCAGA1200.042.4999965
TATCAAC20050.039.6384052
ATGGTTC559.5351446E-538.6363686
TACATGA450.001659950437.777782
GGTATCA8550.036.7836271
GTCCAGC601.5910952E-435.4166641
GACAGGA1451.8189894E-1235.1724131
ATCAACG22950.034.8148163
CGACTTT1600.034.531253
TCAACGC23450.034.0724954
TCAGATG1254.4201443E-1034.0000047
GTCGACT1650.033.4848481
Produced by FastQC (version 0.11.5)