FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168137
Sequences flagged as poor quality0
Sequence length91
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA33381.985285808596561No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA18861.1217043244497047No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17861.0622290156241636No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA16680.9920481512100251No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.9795583363566616No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.8284910519397871No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT10630.6322225328155016No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.6310330266389907No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA9590.570368211636939No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA8600.5114876558996532No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA8360.49721358178152336No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA7460.44368580383853645No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.37766821104218584No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.3413882726586058No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA5480.3259246923639651No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5050.30035030956898245No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG4070.24206450691995218No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.23968549456693053No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA4000.23790123530216428No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3720.22124814883101282No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.21708487721322492No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC3550.21113734633067083No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAA3540.21054259324241542No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3470.20637932162462752No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC3410.20281080309509508No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA3400.20221605000683968No Hit
ATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGG3390.20162129691858427No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA3390.20162129691858427No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.19864753147730718No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.19626851912428553No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA3200.19032098824173144No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA3180.18913148206522062No Hit
TTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATAC3080.1831839511826665No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.17783117338836782No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.17366790177057995No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA2750.16355709927023795No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC2750.16355709927023795No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.15879907456419468No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTG2650.15760956838768386No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA2630.15642006221117305No Hit
GTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAA2600.1546358029464068No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.15166203750512974No Hit
GTGGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACAT2550.15166203750512974No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTG2540.15106728441687434No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAA2510.1492830251521081No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.143335494269554No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.14155123500478778No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.13917222265176613No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.13084567941619038No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2120.12608765471014707No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.1213296300041038No Hit
ATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAA2020.12014012382759298No Hit
GATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCT2010.11954537073933756No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1970.11716635838631594No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAA1960.11657160529806052No Hit
TACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAA1930.11478734603329428No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGCTGTCTCT1920.11419259294503886No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.11359783985678346No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCCT1860.11062407441550641No Hit
GTGGTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTT1840.10943456823899558No Hit
GATATACGCATTGTGGCAGTACATCAAGCGAAAAAAAAAAAAAAAAAAAA1790.10646080279771854No Hit
ATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAA1780.10586604970946312No Hit
CAGTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTT1750.10408179044469688No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.10348703735644146No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCCTGTCTCTT1740.10348703735644146No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG150.001028020985.000011
GCGTCAG357.723611E-872.857154
GTGGTAT8550.067.602341
AGCGTCA401.9467188E-763.753
TCTTGAT200.003218695863.752
TATGCAT200.003218695863.755
TGCATTG200.003218695863.757
TACTGGC200.003218695863.757
ATGCATT200.003218695863.756
GACCTTA554.0563464E-1061.8181847
TGGTATC9750.059.282052
ATGACCT454.3945693E-756.6666685
TCACCGC250.00778474751.04
CATTGGA250.00778474751.02
TATATGC250.00778474751.03
AGGCGTA250.00778474751.03
TACATGA509.094201E-751.02
GTCAGAT509.094201E-751.06
TCAGATG509.094201E-751.07
GCAGCGT509.094201E-751.01
Produced by FastQC (version 0.11.5)