FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168137
Sequences flagged as poor quality0
Sequence length61
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13340.7934006197327179No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7730.45974413722143254No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA7290.43357500133819443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6410.3812367295717183No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.23909074147867512No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA3680.21886913647799117No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTATACACATCTGA3560.21173209941892623No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATCTGACGCTGCCG3300.19626851912428553No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTCTCTTATACACA2960.1760469141236016No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG2850.16950463015279207No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGA2480.14749876588734187No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAA2270.13500895103397825No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA2270.13500895103397825No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCTGTCTCTTATACA2180.12965617323967954No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAA2180.12965617323967954No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.12251913618061464No Hit
ATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACA2060.12251913618061464No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.12192438309235921No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAA2000.11895061765108214No Hit
GTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA1970.11716635838631594No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGCTGTCTCTTA1880.11181358059201724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGA150.005773322755.00416623
CGAGTCC150.005773322755.00416627
CGACGAC150.005773322755.00416655
TGTTCCC150.005773322755.00416610
GTGTATC150.005773322755.0041661
TGCTCAC150.005773322755.00416611
ATGCGAC150.005773322755.00416621
ACACTAT150.005773322755.00416635
ACGAGTC150.005773322755.00416626
TACACTA150.005773322755.00416634
GGAGGAC150.005773322755.00416655
GTGGTAT9850.049.6991961
TTAAACG453.243258E-948.8925922
TGCTTAA351.5630503E-647.14642319
TGGTATC10950.044.6932832
TAGAGAA257.8343635E-444.003339
TCTTGGA257.845856E-443.99024241
ATCCAAT257.845856E-443.99024216
GCTTAAA451.7909952E-742.78101720
GACAGGA403.9177376E-641.2531241
Produced by FastQC (version 0.11.5)