FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12610
Sequences flagged as poor quality0
Sequence length91
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1591.260904044409199No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA1010.8009516256938937No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.8009516256938937No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.6026962727993657No Hit
CCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.5154639175257731No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT530.4203013481363997No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.38065027755749403No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.38065027755749403No Hit
GTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA470.37272006344171293No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA450.3568596352101507No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGG440.3489294210943696No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.317208564631245No Hit
GTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.30134813639968283No Hit
CCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.2775574940523394No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACAAGAGGC350.2775574940523394No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT310.2458366375892149No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGGCGT300.23790642347343377No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG260.2061855670103093No Hit
AAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAA260.2061855670103093No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.19825535289452814No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.19032513877874702No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.19032513877874702No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAA240.19032513877874702No Hit
CCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAA230.1823949246629659No Hit
GTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAA210.16653449643140364No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA200.1586042823156225No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.1586042823156225No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.15067406819984142No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT180.14274385408406026No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAAAAAA180.14274385408406026No Hit
GGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAAAAAAAA180.14274385408406026No Hit
ATACATGACCTTATGGGACTTTCCTACTTGACCTGTCTCTTATACACATC180.14274385408406026No Hit
CAAGTAGGAAAGTCCCATAAGGTCATGTATTGGGCAAAAAAAAAAAAAAA180.14274385408406026No Hit
GGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA180.14274385408406026No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.13481363996827914No Hit
GTCATGTATTGGGCATAATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAA170.13481363996827914No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGGGTGGCGG170.13481363996827914No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.13481363996827914No Hit
GTCAAGTAGGAAAGTCCCATAAGGTCATGTATCTGTCTCTTATACACATC160.126883425852498No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.126883425852498No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGT140.11102299762093576No Hit
AGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCAA140.11102299762093576No Hit
CCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAAAAA130.10309278350515465No Hit
GTCCCATAAGGTCATGTATTGGGCATAATGCCAAAAAAAAAAAAAAAAAA130.10309278350515465No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10309278350515465No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT130.10309278350515465RNA PCR Primer, Index 33 (95% over 22bp)
GTGTATAAGAGACAGATACACATCTCCGAGCCCACGAGACAAGAGGCAAT130.10309278350515465No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGACC250.007619544451.04
TGACCTT250.007619544451.06
ACATGAC250.007619544451.03
GATATAC508.435509E-751.01
ATGACCT250.007619544451.05
TACATGA250.007619544451.02
CCTTATG250.007619544451.09
ACCTTAT250.007619544451.08
GACCTTA250.007619544451.07
ATACATG250.007619544451.01
TCGACTT354.6463066E-448.5714262
GACTTTT354.6463066E-448.5714264
CGACTTT354.6463066E-448.5714263
GTCAAGT408.972257E-442.51
GTATCAA809.84619E-942.51
ATATACG602.9627736E-642.4999962
ACGCATT655.136808E-639.2307666
CGCATTG655.136808E-639.2307667
TCAAGTA450.001601398337.777782
GTGTATA450.001601398337.777781
Produced by FastQC (version 0.11.5)