FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005411830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832
Sequences flagged as poor quality0
Sequence length91
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA100.5458515283842794No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA90.49126637554585156No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.32751091703056767No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA50.2729257641921397No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA50.2729257641921397No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA50.2729257641921397No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA50.2729257641921397No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT40.21834061135371177No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA40.21834061135371177No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.21834061135371177No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.21834061135371177No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.21834061135371177No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.21834061135371177No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC30.16375545851528384No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16375545851528384No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16375545851528384No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16375545851528384No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA30.16375545851528384No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG30.16375545851528384No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC30.16375545851528384No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT30.16375545851528384No Hit
GTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAA30.16375545851528384No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.16375545851528384No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.16375545851528384No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA30.16375545851528384No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTACTCCATGA30.16375545851528384No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
GTGGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACAT20.10917030567685589No Hit
ATTCCAATCCATGCCATTCCACTCGTGTTGATTCCATTCTTTCCATTCCA20.10917030567685589No Hit
GTTCCATTCCATTCAAGTTGAATCCATTCCATTGCATTGCATTCCATTCG20.10917030567685589No Hit
CCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAG20.10917030567685589No Hit
GTTTTCCACTTCAAACTTCTATCTTCTCCAAATTGTTTCATCCTACCACT20.10917030567685589No Hit
GTTGTGGACCTGGAGGCGAGAAATGTCCCTGCAACGGGCCAAAATCCTGC20.10917030567685589No Hit
GCAGTACACCATCTATCTGGATTGATGTTACAGCGGCACCAGGGAGTGAG20.10917030567685589No Hit
GGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA20.10917030567685589No Hit
CAATTATACACAATAAAAAAGGACCGAAACCAACAGAAAACAAGAAAGAG20.10917030567685589No Hit
GGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGC20.10917030567685589No Hit
TGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACC20.10917030567685589No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
AAATGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTG20.10917030567685589No Hit
ACTTAACGCTAAGCAATATCTTAGTAACCTATTGACTATATTTTTTAAGT20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC20.10917030567685589No Hit
GGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT20.10917030567685589No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG20.10917030567685589No Hit
GAGAACACTCGTGTAGGCCAAGTTGCAAACTATGATAAACTTACACTTGA20.10917030567685589No Hit
ATCTTACACTAATTTGAACTCTTAAAGATTGCTGCTTTTTTTTGATATTG20.10917030567685589No Hit
GATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCG20.10917030567685589No Hit
GTCAGGATGGCAGTATGCCTGCTCACATGTTGGTCAAACTGTCACTAGGG20.10917030567685589No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
ATGATAAACTTACACTTGATGTTTGGACTGATGGAAGCACTGGACCGAAA20.10917030567685589No Hit
GGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAAT20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA20.10917030567685589No Hit
GCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAG20.10917030567685589No Hit
GGTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT20.10917030567685589No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT20.10917030567685589No Hit
TCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGTAGATGTACTGTCAAGTAGGAAAGTCCCAA20.10917030567685589No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC20.10917030567685589No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
GTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAA20.10917030567685589No Hit
ATTCTATTCCGTACCATTCCATTCCATTCCATTCCATGCCATTCCACTCG20.10917030567685589No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.10917030567685589No Hit
GTGCAAAACAAGGTTATCAATAATTGCCCCGTACTCTGCGTTGATCTGTC20.10917030567685589No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.10917030567685589No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG20.10917030567685589No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.10917030567685589No Hit
CCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGC20.10917030567685589No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA20.10917030567685589No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAA20.10917030567685589No Hit
GATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTT20.10917030567685589No Hit
ATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTG20.10917030567685589No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG20.10917030567685589No Hit
ACTTTAAGCTGTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTT20.10917030567685589No Hit
AGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAG20.10917030567685589No Hit
CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA20.10917030567685589No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.10917030567685589No Hit
ATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTCAAGCTC20.10917030567685589No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.10917030567685589No Hit
AACGTATTCACCGCGACATTCTGATCCGCGATTACTAGCGATTCCGACTT20.10917030567685589No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAA20.10917030567685589No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)