FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832
Sequences flagged as poor quality0
Sequence length61
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.38209606986899564No Hit
GTCTAACGAAGAGAGCTTAAATGAAAGTGTGAATGGAGGATTGCCAACAGGGTTGACAAAG30.16375545851528384No Hit
GGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTG30.16375545851528384No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
GATTTCAAGGTCTTTTAATCACCTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATT20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGA20.10917030567685589No Hit
GAGTACGGGGCAATTATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACG20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
GTGCAATCTTGGGTCACTGCAACCTCCGCCTCCCAGGTTCAAGCGCTTCTCCTGCCTCAGC20.10917030567685589No Hit
ATCAACGCAGAGTACGGGGCAATTATTGATAACCTTGTTTTGCACCTGTCTCTTATACACA20.10917030567685589No Hit
GAGTACGGGATACCAGCTTATTCAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
GGTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG20.10917030567685589No Hit
ATCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAG20.10917030567685589No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
AGGCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCAAGGTCTTTTAATCACCTTCG20.10917030567685589No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAG20.10917030567685589No Hit
GAGTACGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGC20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCACTGTCTCTTATA20.10917030567685589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAA20.10917030567685589No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10917030567685589No Hit
GGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACA20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC20.10917030567685589No Hit
TACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAAATTGTTTCATCCTAC20.10917030567685589No Hit
GTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGA20.10917030567685589No Hit
CCTTTTATCTTTTCCTTCCTCATGCTACTCTTCTAAGTCTTCATATTTTCTCTTAAAATCT20.10917030567685589No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA20.10917030567685589No Hit
TGGTACAACTCTGGGTAACTCCTTACGTCGTATCCTCTTATCCTCACTCCCTGGTGCCGCT20.10917030567685589No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA20.10917030567685589No Hit
GGGTTAGAAAAAGGGGGATAAAACAAAGATAAATAAAGGAATGAAAGAAATAGGAGAAAAA20.10917030567685589No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10917030567685589No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGT20.10917030567685589No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA20.10917030567685589No Hit
CCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACA20.10917030567685589No Hit
GTATCAACGCAGAGTACGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA20.10917030567685589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA355.6892488E-539.2857131
TATCAAC401.2512942E-434.3749962
TCAACGC558.1325765E-425.04
ATCAACG558.1325765E-425.03
CAACGCA558.1325765E-425.05
Produced by FastQC (version 0.11.5)