FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427510
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA108720.7616058731637607No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT58440.409384172440123No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58090.4069323507365973No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA57230.40090787455079124No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45640.31971755013975384No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.29253735525495445No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA39230.2748141869408971No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT37230.2608037772064644No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.1702264782733571No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA24020.16826502091053652No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA19890.13933352480893305No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA18760.13141764330897857No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17250.12083978395948189No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.10038458574721018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT31850.055.124721
TGGTATC32450.054.2231752
GCGTCAG3900.052.308944
AGCGTCA4200.047.560663
GTATCAA114150.040.2210731
GGTATCA59350.039.3956031
ACGGTCA450.001662700237.7800036
ATATCGC805.411421E-737.1896936
GCAGCGT5650.036.1159131
ATATACG74250.035.088382
TATCAAC132800.034.9161222
GATATAC72450.034.85411
GCGTACG500.00278888534.0008134
ATACGCA77100.033.7362144
CGTCAGA6150.033.1726885
CGCATTG78150.033.012187
TATACGC78900.032.91273
TACGCAT78800.032.7398765
GCATTGT78700.032.2414178
ATCAACG146700.031.8105753
Produced by FastQC (version 0.11.5)