FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427510
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66530.4660562798159032No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42200.29561964539652963No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22490.15754705746369552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT41050.045.184571
TGGTATC43150.042.9674842
ACGCGGT852.0008883E-1135.58875754
TACGCGG658.395E-833.84665353
ACGCAAC801.5019396E-830.93903545
CGTAGAC400.007873227.50040453
ACACGCC400.00787454627.49944141
GGTCCGT400.00787589127.4984766
ATTCGAC400.00787589127.49847614
TCGGCGC400.00787589127.49847628
CTACGCG805.1834286E-727.49847652
TCTGTCG1203.6379788E-1127.4984768
AACCGGC1057.2668627E-926.18902626
TAGGCCG1057.2668627E-926.1890264
GTATCAA150750.025.9588641
TGAACCG1201.0877557E-925.20693824
GGTATCA78350.025.078561
GGCGTCC550.001226846124.9994941
ACCGGCT1001.3108729E-724.74862927
GACCTTA2350.024.5731077
Produced by FastQC (version 0.11.5)