FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241158
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA64460.5193537003346874No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44340.3572470225386292No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.32034599946179293No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA38520.31035532945845734No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.28191414791670355No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29380.2367144231435482No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT27040.21786106200822136No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA22560.18176573812520244No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.13551860440008445No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA15970.12867016125263667No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC14260.11489270503835934No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC13340.10748027245523939No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA12960.10441861551873331No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.10175980817913592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19250.059.1729551
GCGTCAG2950.056.1858334
TGGTATC21350.053.7464832
AGCGTCA3400.047.499483
GCAGCGT3350.046.9435651
GTATCAA88550.041.9509741
GGTATCA46350.039.8895151
CGTCAGA4600.037.8815465
GATATAC43850.037.317311
CAGCGTC4450.037.2467842
ATATACG46150.037.112272
TATCAAC105050.035.803992
ACGCTAG955.1348252E-835.7919651
ATACGCA48100.035.607724
CGCATTG47850.035.3510937
TATACGC48800.034.9227753
GCATTGT50600.033.4311838
TACGCAT53600.032.5102845
ACGCATT52450.032.4127736
ATCAACG118350.032.0676733
Produced by FastQC (version 0.11.5)