FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241158
Sequences flagged as poor quality0
Sequence length61
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46620.37561696415766566No Hit
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23610.190225579660285No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14530.11706809286166628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28750.047.0045741
TGGTATC31050.043.3192672
ATCGGAT504.125177E-738.4996616
GGAACGT704.087269E-935.35540813
CTAGGCC401.9021814E-434.3733144
AGACCCG658.398274E-833.8444946
ACTCGCC601.7174752E-632.08305420
ACCGCGA701.6091872E-731.42829524
GGCGACT701.6091872E-731.42829520
GACCCGG701.609751E-731.427037
TACGGCC453.795916E-430.55528640
CGCGACA653.206189E-629.61512426
CCATCGG653.2071948E-629.6139314
TCCGCGA752.9457078E-729.33307639
ATCCGCG752.9457078E-729.33307638
CACCGCG705.707805E-627.4997623
ACTTACC805.180391E-727.49975824
AGATACG705.709593E-627.49865211
TTAGACG1004.318281E-927.4986524
CGGCTTC507.0342567E-427.4986525
Produced by FastQC (version 0.11.5)