FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031648
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA62910.6098010174012841No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36940.35806786811005303No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.3430433636279041No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA32880.31871335959552094No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.24746812866404044No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.24262151431496012No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT22830.2212964111790068No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA21760.21092465647197492No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.14152113899314495No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA14410.13967942554049445No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11470.11118133316790223No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA11460.11108440088092063No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.1012942398957784No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA10400.10080957846087038No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA10340.10022798473898074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19000.060.181281
TGGTATC21300.054.8748972
GCGTCAG2750.051.0037234
ACCGTTA354.82387E-448.5773288
AGCGTCA2800.048.5749743
GCAGCGT3350.041.8727871
GTATCAA73050.039.8596151
CGTCAGA3550.038.3126565
CGTATCA450.001661579537.7842031
GGTATCA38600.036.780861
TCAGATG3900.035.9659087
ATATACG38000.035.4565352
GATATAC37500.035.252661
TATCAAC85350.034.510432
TATACGC40250.033.2632983
ATACGCA41250.032.4569134
CAGCGTC4200.032.3833162
CGCATTG41200.032.2915427
TACGCAT43050.031.593485
ATCAACG95350.030.980243
Produced by FastQC (version 0.11.5)