FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553456
Sequences flagged as poor quality0
Sequence length91
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAA94801.712873290740366No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62241.1245699748489493No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT56091.0134500303547167No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.856617328206759No Hit
GATATACGCATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAA43940.7939203839148913No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42390.765914544245613No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAA41440.7487496747708942No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT37400.675753808794195No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAA29500.5330143678991645No Hit
GATATACGCATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAA26970.48730161024543955No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.4383365615333468No Hit
GAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA23060.4166546211442283No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.36443728137376774No Hit
CATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20050.3622690873348559No Hit
GGTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCA18390.3322757364632419No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.2759026914515336No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14370.2596412361596947No Hit
GGTATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAA14250.25747304212078287No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.2482582174554075No Hit
TATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAG12880.23271949350987248No Hit
TATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12810.23145471365384063No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.2159159897083056No Hit
TATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAA11310.2043522881674424No Hit
TATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAA11160.20164204561880258No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT10580.19116244109739527No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.18140556792229193No Hit
ACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.17959873955653205No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9760.17634644849816428No Hit
ATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAA9700.17526235147870833No Hit
CATTGTGGCAGTACATCAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9390.1696611835448527No Hit
ATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8710.15737475065768552No Hit
GAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAA8460.15285767974328582No Hit
ATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGG8360.15105085137752597No Hit
ATCAACGCAGAGTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAA8040.14526900060709433No Hit
ATTGATAACCTTGTTTTGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA7810.1411132953658466No Hit
GTACGGGCCAGATATACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAA7780.14057124685611866No Hit
GTGGTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTT7730.1396678326732387No Hit
GCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC7530.13605417594171892No Hit
GTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTC7470.134970078922263No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCCTGTCTCTTATACACATC7420.13406666473938308No Hit
GTATCAACGCAGAGTCGAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.13298256771992714No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.1324405192101992No Hit
ATGTACTGTCAAGTAGGAAAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAA7210.1302723251712873No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.12991095949813536No Hit
CCCTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGA7050.12738139978607152No Hit
GCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATACCTGTCTCTTA6990.1262973027666156No Hit
GTATCAACGCAGAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAA6970.12593593709346362No Hit
GTACGGGATACCAGCTTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.12376774305455177No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.11798589228412014No Hit
TTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATAC6500.1174438437743922No Hit
CATTGTGGCAGTACATCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6460.11672111242808823No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCAGTACCTGTC6330.11437223555260038No Hit
CTTGATGTACTGCCACAATGCGTATATCTGGCCCGTACTCTGCGTTGATA6210.11220404151368853No Hit
GAGTACGGGATACCAGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.10804833627244081No Hit
GTATCAACGCAGAGTACGGGCCAGATATACGCATTGTGGCCTGTCTCTTA5950.10750628776271284No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5830.10533809372380099No Hit
TACGCATTGTGGCAGTACATCAAGGGAAAAAAAAAAAAAAAAAAAAAAAA5820.105157410887225No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.10009829146309734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT27300.064.952371
AGCGTCA1500.062.332933
CCTGAAC356.606522E-660.7138988
TGGTATC30450.059.0390322
CAGTGGT2150.053.40071
TACTAGG250.00779432650.9996722
TACCGCA250.00779432650.9996727
GCGTCAG1850.050.540224
CTGAACA505.4485005E-542.499739
ATCCGAT255.4520468E-542.4997320-21
CGAATTG409.315505E-442.499737
CGTCAGA2250.041.555295
GTATCAA117850.039.9429051
GGTATCA61250.039.3639451
CCCTTGA2400.037.2074281
GTCAGAT2550.036.666436
GCAGCGT2600.035.9808121
GCTATCA601.5894466E-435.4356461
TCCGATA301.595552E-435.4164422-23
GATATAC61300.035.3778421
Produced by FastQC (version 0.11.5)